hek293t cells atcc cat (ATCC)
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hek293t cells atcc cat
Hek293t Cells Atcc Cat, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 12132 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/hek293t+atcc+cat/293T%2F17/10__1016_slash_j__isci__2026__116022-200-114-116
Average 99 stars, based on 12132 article reviews
Hek293t Cells Atcc Cat, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 12132 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/hek293t+atcc+cat/293T%2F17/10__1016_slash_j__isci__2026__116022-200-114-116
Average 99 stars, based on 12132 article reviews
hek293t cells atcc cat - by Bioz Stars,
2026-09
99/100 stars
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REAGENT or RESOURCE SOURCE IDENTIFIER Near-infrared lipophilic carbocyanine tracer DiR KeyGEN BioTECH Cat#KGE2603-5 Rhodamine MACKLIN Cat#R817228 ICG MACKLIN Cat#I953656 Luciferase substrate Vazyme Cat#DD1210 H2DCFDA MedChemExpress Cat#HY-D0940 Triton X-100 Sigma-Aldrich Cat#93443 M-CSF Yeasen Cat#91109ES10 GM-CSF Diane Biologics Cat#PDEM100050 IL4 protein Bioworld Cat#BK0246 DMF MedChemExpress Cat#HY-17363 DSF MedChemExpress Cat#HY-B0240 TAK-242 Selleck Cat#S7455 TH1020 MedChemExpress Cat#HY-116961 anti-PD1 antibody MedChemExpress Cat#A2122 Critical commercial assays Uniclone One-Step Seamless Cloning Kit Genesand Cat#SC612 T4 DNA ligase Sangon Biotech Cat#B600511-0001 FreeZol Reagent Vazyme Cat#R711-01 Reverse transcription kit Thermo Scientific Cat#M16315 AceQ qPCR SYBR Green Master Mix Vazyme Cat#Q221-01 permeabilization and fixation kit BD Biosciences Cat#555028 LDH Cytotoxicity Assay Kit Solarbio Cat#BC0680 ABplex Mouse Cytokine 15-Plex Assay Kit Abclonal Cat#RK05203 HMGB1 ELISA kit BYabscience Cat#BYHS504658 Deposited data Data files for RNA-sequencing This paper GEO: GSE291580 Experimental models: Cell lines 4T1 ATCC Cat#CRL-2539 H22 BLUEFBIO Cat#BFN608007276 A20 ATCC Cat#TIB-208 Panc02 Servicebio Cat Mutagenesis:Article Title: CD5L promotes phagocytic removal of amyloid β oligomers and improves cognitive function in a mouse model of Alzheimer's disease. Article Snippet: The virus solution was stereotaxically injected into the dorsal hippocampus (Anterior-Posterior -2.0 mm; Medial-Lateral +1.5 mm; DorsalVentral +3.0 mm) using a 30 G needle and a stereotaxic injector (Muromachi Kikai Co., Ltd., Japan).The mice were sacrificed .. REAGENT or RESOURCE SOURCE IDENTIFIER Experimental models: Cell lines Hamster: CHO-S Thermo Fisher Scientific Cat#A1155701 Human: Software:Article Title: CD5L promotes phagocytic removal of amyloid β oligomers and improves cognitive function in a mouse model of Alzheimer's disease. Article Snippet: The virus solution was stereotaxically injected into the dorsal hippocampus (Anterior-Posterior -2.0 mm; Medial-Lateral +1.5 mm; DorsalVentral +3.0 mm) using a 30 G needle and a stereotaxic injector (Muromachi Kikai Co., Ltd., Japan).The mice were sacrificed .. REAGENT or RESOURCE SOURCE IDENTIFIER Experimental models: Cell lines Hamster: CHO-S Thermo Fisher Scientific Cat#A1155701 Human: Immunohistochemistry:Article Title: CD5L promotes phagocytic removal of amyloid β oligomers and improves cognitive function in a mouse model of Alzheimer's disease. Article Snippet: The virus solution was stereotaxically injected into the dorsal hippocampus (Anterior-Posterior -2.0 mm; Medial-Lateral +1.5 mm; DorsalVentral +3.0 mm) using a 30 G needle and a stereotaxic injector (Muromachi Kikai Co., Ltd., Japan).The mice were sacrificed .. REAGENT or RESOURCE SOURCE IDENTIFIER Experimental models: Cell lines Hamster: CHO-S Thermo Fisher Scientific Cat#A1155701 Human: Article Title: Targeting CTGF overcomes resistance to CSF1R inhibitors by preventing CAF activation in colorectal cancer. Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER BLZ945 MCE Cat#HY-12768 Pamrevlumab (anti-CTGF) Selleck Cat#A2042 EDHB sigma Cat# E24859 LY294002 MCE Cat#HY-10108 740-Y-P MCE Cat#HY-P0175 Anti-PD-1 monoclonal antibody selleck Cat#A2122 Matrigel VivoMatter Biotech Cat#VM002-PRF-10 Organoid Complete Medium VivoMatter Biotech Cat#RSQ-OCM1102 Fetal Bovine Serum Umedium Cat#3021A Critical commercial assays Masson’s Trichrome Stain Kit Solarbio Cat#G1340 Sirius Red Stain Kit Solarbio Cat#G1472 Mouse CTGF ELISA KIT Shanghai Enzyme-linked Biotechnology Cat#ml037697 Human CTGF ELISA KIT Shanghai Enzyme-linked Biotechnology Cat#ml025961 Omni-Easy TM One-step Color PAGE Gel Rapid Preparation Kit EpiZyme Cat#PG212 Multiplex Fluorescent Immunohistochemistry Staining Kit absin Cat#abs50029 PrimeScript TM FAST RT reagent Kit with gDNA Eraser Takara Cat#RR092A Talent qPCR PreMix TIANGEN Cat#FP209 Deposited data Bulk RNA-seq raw data This paper GSA:PRJCA040243 IMC raw data This paper GSA:OMIX012148 Experimental models: Cell lines CT26 Pricella Biotechnology Cat#CL-0071 CMT93 Pricella Biotechnology Cat#CL-0920 NIH/3T3 Pricella Biotechnology Cat#CL-0171 HCT-8 Pricella Biotechnology Cat#CL-0098 Caco2 Pricella Biotechnology Cat#CL-0050 HCT15 Pricella Biotechnology Cat#CL-0097 HT29 Pricella Biotechnology Cat#CL-0118 HCT116 Pricella Biotechnology Cat# Real-time Polymerase Chain Reaction:Article Title: CD5L promotes phagocytic removal of amyloid β oligomers and improves cognitive function in a mouse model of Alzheimer's disease. Article Snippet: The virus solution was stereotaxically injected into the dorsal hippocampus (Anterior-Posterior -2.0 mm; Medial-Lateral +1.5 mm; DorsalVentral +3.0 mm) using a 30 G needle and a stereotaxic injector (Muromachi Kikai Co., Ltd., Japan).The mice were sacrificed .. REAGENT or RESOURCE SOURCE IDENTIFIER Experimental models: Cell lines Hamster: CHO-S Thermo Fisher Scientific Cat#A1155701 Human: Article Title: Targeting CTGF overcomes resistance to CSF1R inhibitors by preventing CAF activation in colorectal cancer. Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER BLZ945 MCE Cat#HY-12768 Pamrevlumab (anti-CTGF) Selleck Cat#A2042 EDHB sigma Cat# E24859 LY294002 MCE Cat#HY-10108 740-Y-P MCE Cat#HY-P0175 Anti-PD-1 monoclonal antibody selleck Cat#A2122 Matrigel VivoMatter Biotech Cat#VM002-PRF-10 Organoid Complete Medium VivoMatter Biotech Cat#RSQ-OCM1102 Fetal Bovine Serum Umedium Cat#3021A Critical commercial assays Masson’s Trichrome Stain Kit Solarbio Cat#G1340 Sirius Red Stain Kit Solarbio Cat#G1472 Mouse CTGF ELISA KIT Shanghai Enzyme-linked Biotechnology Cat#ml037697 Human CTGF ELISA KIT Shanghai Enzyme-linked Biotechnology Cat#ml025961 Omni-Easy TM One-step Color PAGE Gel Rapid Preparation Kit EpiZyme Cat#PG212 Multiplex Fluorescent Immunohistochemistry Staining Kit absin Cat#abs50029 PrimeScript TM FAST RT reagent Kit with gDNA Eraser Takara Cat#RR092A Talent qPCR PreMix TIANGEN Cat#FP209 Deposited data Bulk RNA-seq raw data This paper GSA:PRJCA040243 IMC raw data This paper GSA:OMIX012148 Experimental models: Cell lines CT26 Pricella Biotechnology Cat#CL-0071 CMT93 Pricella Biotechnology Cat#CL-0920 NIH/3T3 Pricella Biotechnology Cat#CL-0171 HCT-8 Pricella Biotechnology Cat#CL-0098 Caco2 Pricella Biotechnology Cat#CL-0050 HCT15 Pricella Biotechnology Cat#CL-0097 HT29 Pricella Biotechnology Cat#CL-0118 HCT116 Pricella Biotechnology Cat# Article Title: Controlled pyroptosis of engineered macrophages enables biphasic antitumor via the release of oncolytic bacteria and inflammatory signals. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies FITC-conjuagted-anti-MHCII BD Biosciences Cat#562009; RRID:AB_10893593 FITC-conjuagted-anti-CD3 BD Biosciences Cat#561801; RRID:AB_10895388 FITC-conjuagted-anti-CD11b BD Biosciences Cat#561684; RRID:AB_10893796 FITC-conjuagted-anti-CD44 BD Biosciences Cat#553133; RRID:AB_2076224 PE-Cy7-conjuagted-anti-CD11c BD Biosciences Cat#558079; RRID:AB_647251 PE-Cy7-conjuagted-anti-CD8 BD Biosciences Cat#552877; RRID:AB_394506 PE-conjuagted-anti-CD8 BD Biosciences Cat#554857; RRID:AB_395558 PE-conjuagted-anti-Foxp3 BD Biosciences Cat#563101; RRID:AB_2738006 PerCP-Cy5.5-conjuagted-anti-CD4 BD Biosciences Cat#550954; RRID:AB_393977 BV421-conjuagted-anti-F4/80 BD Biosciences Cat#565411; RRID:AB_2734779 BV510-conjuagted-anti-CD45 BD Biosciences Cat#740131; RRID:AB_2739888 APC-conjuagted-anti-CD62L BD Biosciences Cat#553152; RRID:AB_398533 APC-conjuagted-anti-F4/80 BD Biosciences Cat#566787; RRID:AB_2869866 APC-conjuagted-anti-CD11b BD Biosciences Cat#553312; RRID:AB_398535 APC-conjuagted-anti-CD206 Thermo Fisher Scientific Cat#17-2061-82; RRID:AB_2637420 APC-conjuagted-anti-GzmB Thermo Fisher Scientific Cat#17-8898-82; RRID:AB_2688068 PE-conjuagted-anti-CD86 Thermo Fisher Scientific Cat#12-0862-82; RRID:AB_465768 OVA257-264 (SIINFEKL) peptide bound to H-2Kb Monoclonal Antibody-APC Thermo Fisher Scientific Cat#17-5743-80; RRID:AB_1311288 FITC-conjuagted-anti-CD45.1 Biolegend Cat#110705; RRID:AB_313494 Anti-GSDMD rabbit mAb Abcam Cat#ab209845; RRID:AB_2783550 Anti-tubulin rabbit mAb ABclonal Cat#A12289; RRID:AB_2861647 HRP-conjugated Goat anti-Rabbit IgG ABclonal Cat#AS014; RRID:AB_2769854 Bacterial and virus strains Salmonella Typhimurium VNP20009 (VNP) ATCC Cat#202165 VNP-2 (VNP strains constitutively expresses RFP) This paper N/A DLV (VNP strains defective in lon gene) This paper N/A DLV-1(DLV strains constitutively expresses sfGFP) This paper N/A DLV-2 (DLV strains constitutively expresses RFP) This paper N/A DLV-3(DLV strains constitutively expresses luxCDABE) This paper N/A DLV-4(DLV strains constitutively expresses Nirfp) This paper N/A DLV-5(DLV strains expresses RFP with the aid of the sifB promoter) This paper N/A DLV-6(DLV strains expresses lysE with the aid of the sifB promoter) This paper N/A Chemicals, peptides, and recombinant proteins Mycoplasma removal reagent Yeasen Cat#40607ES03 transfection reagents Yeasen Cat#40802ES08 fixable viability dye BD Cat#564407 Hoechst dye KeyGEN BioTECH Cat#KGA1804-10 (Continued on next page) 18 Cell Reports 45, 116918, February 24, 2026 .. REAGENT or RESOURCE SOURCE IDENTIFIER Near-infrared lipophilic carbocyanine tracer DiR KeyGEN BioTECH Cat#KGE2603-5 Rhodamine MACKLIN Cat#R817228 ICG MACKLIN Cat#I953656 Luciferase substrate Vazyme Cat#DD1210 H2DCFDA MedChemExpress Cat#HY-D0940 Triton X-100 Sigma-Aldrich Cat#93443 M-CSF Yeasen Cat#91109ES10 GM-CSF Diane Biologics Cat#PDEM100050 IL4 protein Bioworld Cat#BK0246 DMF MedChemExpress Cat#HY-17363 DSF MedChemExpress Cat#HY-B0240 TAK-242 Selleck Cat#S7455 TH1020 MedChemExpress Cat#HY-116961 anti-PD1 antibody MedChemExpress Cat#A2122 Critical commercial assays Uniclone One-Step Seamless Cloning Kit Genesand Cat#SC612 T4 DNA ligase Sangon Biotech Cat#B600511-0001 FreeZol Reagent Vazyme Cat#R711-01 Reverse transcription kit Thermo Scientific Cat#M16315 AceQ qPCR SYBR Green Master Mix Vazyme Cat#Q221-01 permeabilization and fixation kit BD Biosciences Cat#555028 LDH Cytotoxicity Assay Kit Solarbio Cat#BC0680 ABplex Mouse Cytokine 15-Plex Assay Kit Abclonal Cat#RK05203 HMGB1 ELISA kit BYabscience Cat#BYHS504658 Deposited data Data files for RNA-sequencing This paper GEO: GSE291580 Experimental models: Cell lines 4T1 ATCC Cat#CRL-2539 H22 BLUEFBIO Cat#BFN608007276 A20 ATCC Cat#TIB-208 Panc02 Servicebio Cat other:Article Title: Vorapaxar enhanced mitochondria-associated ferroptosis primes cancer immunotherapy via targeting FOXO1/HMOX1 axis. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER RIPA lysis buffer Beyotime Cat#P0013C BCA reagent Beyotime Cat#P0011 Clarity Western ECL Substrates BIO-RAD Cat#1705061 Goat serum blocking solution ZSGB-Bio Cat#ZLI-9056 Critical commercial assays Lipid Peroxidation Assay Kit Merck Cat#MAK085 GSH and GSSG assay kit Beyotime Cat#S0053 Glutathione Peroxidase Assay Abcam Cat#ab102530 Human Coenzyme Q10 ELISA Kit Cusabio Cat#CSB-E14081h ATP assay kit Beyotime Cat#S0026 Mitochondria Isolation Kit for Cultured Cells MedChemExpress Cat#HY-K1060 Universal Two-Step Polymer Detection System ZSGB-Bio Cat#PV-9000 DAB Substrate Kit ZSGB-Bio Cat#ZLI-9018 Foxp3/Transcription Factor Staining Buffer Set eBioscience Cat#00-5523-00 EZ-LinkTM NHS-LC-LC-Biotin kit Thermo Fisher Scientific Cat#21343 Pierce Zeba Thermo Fisher Scientific Cat#89882 HiScript Q RT SuperMix kit Vazyme Cat#R223-01 Zombie AquaTM Fixable Viability Kit BioLegend Cat#423102 Deposited data RNA-seq data This paper https://zenodo.org/records/16757395 RNA-seq data of inhouse cohort He et al.32 https://doi.org/10.1038/s41392-022-01067-y RNA-seq data of Ribas’s cohort Campbel et al.33 https://github.com/ParkerICI/MORRISON-1-public Experimental models: Cell lines Mouse: Article Title: KIDINS220 and InsP8 safeguard the stepwise regulation of phosphate exporter XPR1. Article Snippet: Article KIDINS220 and InsP8 safeguard the stepwise regulation of phosphate exporter XPR1 Staining:Article Title: Targeting CTGF overcomes resistance to CSF1R inhibitors by preventing CAF activation in colorectal cancer. Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER BLZ945 MCE Cat#HY-12768 Pamrevlumab (anti-CTGF) Selleck Cat#A2042 EDHB sigma Cat# E24859 LY294002 MCE Cat#HY-10108 740-Y-P MCE Cat#HY-P0175 Anti-PD-1 monoclonal antibody selleck Cat#A2122 Matrigel VivoMatter Biotech Cat#VM002-PRF-10 Organoid Complete Medium VivoMatter Biotech Cat#RSQ-OCM1102 Fetal Bovine Serum Umedium Cat#3021A Critical commercial assays Masson’s Trichrome Stain Kit Solarbio Cat#G1340 Sirius Red Stain Kit Solarbio Cat#G1472 Mouse CTGF ELISA KIT Shanghai Enzyme-linked Biotechnology Cat#ml037697 Human CTGF ELISA KIT Shanghai Enzyme-linked Biotechnology Cat#ml025961 Omni-Easy TM One-step Color PAGE Gel Rapid Preparation Kit EpiZyme Cat#PG212 Multiplex Fluorescent Immunohistochemistry Staining Kit absin Cat#abs50029 PrimeScript TM FAST RT reagent Kit with gDNA Eraser Takara Cat#RR092A Talent qPCR PreMix TIANGEN Cat#FP209 Deposited data Bulk RNA-seq raw data This paper GSA:PRJCA040243 IMC raw data This paper GSA:OMIX012148 Experimental models: Cell lines CT26 Pricella Biotechnology Cat#CL-0071 CMT93 Pricella Biotechnology Cat#CL-0920 NIH/3T3 Pricella Biotechnology Cat#CL-0171 HCT-8 Pricella Biotechnology Cat#CL-0098 Caco2 Pricella Biotechnology Cat#CL-0050 HCT15 Pricella Biotechnology Cat#CL-0097 HT29 Pricella Biotechnology Cat#CL-0118 HCT116 Pricella Biotechnology Cat# Enzyme-linked Immunosorbent Assay:Article Title: Targeting CTGF overcomes resistance to CSF1R inhibitors by preventing CAF activation in colorectal cancer. Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER BLZ945 MCE Cat#HY-12768 Pamrevlumab (anti-CTGF) Selleck Cat#A2042 EDHB sigma Cat# E24859 LY294002 MCE Cat#HY-10108 740-Y-P MCE Cat#HY-P0175 Anti-PD-1 monoclonal antibody selleck Cat#A2122 Matrigel VivoMatter Biotech Cat#VM002-PRF-10 Organoid Complete Medium VivoMatter Biotech Cat#RSQ-OCM1102 Fetal Bovine Serum Umedium Cat#3021A Critical commercial assays Masson’s Trichrome Stain Kit Solarbio Cat#G1340 Sirius Red Stain Kit Solarbio Cat#G1472 Mouse CTGF ELISA KIT Shanghai Enzyme-linked Biotechnology Cat#ml037697 Human CTGF ELISA KIT Shanghai Enzyme-linked Biotechnology Cat#ml025961 Omni-Easy TM One-step Color PAGE Gel Rapid Preparation Kit EpiZyme Cat#PG212 Multiplex Fluorescent Immunohistochemistry Staining Kit absin Cat#abs50029 PrimeScript TM FAST RT reagent Kit with gDNA Eraser Takara Cat#RR092A Talent qPCR PreMix TIANGEN Cat#FP209 Deposited data Bulk RNA-seq raw data This paper GSA:PRJCA040243 IMC raw data This paper GSA:OMIX012148 Experimental models: Cell lines CT26 Pricella Biotechnology Cat#CL-0071 CMT93 Pricella Biotechnology Cat#CL-0920 NIH/3T3 Pricella Biotechnology Cat#CL-0171 HCT-8 Pricella Biotechnology Cat#CL-0098 Caco2 Pricella Biotechnology Cat#CL-0050 HCT15 Pricella Biotechnology Cat#CL-0097 HT29 Pricella Biotechnology Cat#CL-0118 HCT116 Pricella Biotechnology Cat# Article Title: Controlled pyroptosis of engineered macrophages enables biphasic antitumor via the release of oncolytic bacteria and inflammatory signals. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies FITC-conjuagted-anti-MHCII BD Biosciences Cat#562009; RRID:AB_10893593 FITC-conjuagted-anti-CD3 BD Biosciences Cat#561801; RRID:AB_10895388 FITC-conjuagted-anti-CD11b BD Biosciences Cat#561684; RRID:AB_10893796 FITC-conjuagted-anti-CD44 BD Biosciences Cat#553133; RRID:AB_2076224 PE-Cy7-conjuagted-anti-CD11c BD Biosciences Cat#558079; RRID:AB_647251 PE-Cy7-conjuagted-anti-CD8 BD Biosciences Cat#552877; RRID:AB_394506 PE-conjuagted-anti-CD8 BD Biosciences Cat#554857; RRID:AB_395558 PE-conjuagted-anti-Foxp3 BD Biosciences Cat#563101; RRID:AB_2738006 PerCP-Cy5.5-conjuagted-anti-CD4 BD Biosciences Cat#550954; RRID:AB_393977 BV421-conjuagted-anti-F4/80 BD Biosciences Cat#565411; RRID:AB_2734779 BV510-conjuagted-anti-CD45 BD Biosciences Cat#740131; RRID:AB_2739888 APC-conjuagted-anti-CD62L BD Biosciences Cat#553152; RRID:AB_398533 APC-conjuagted-anti-F4/80 BD Biosciences Cat#566787; RRID:AB_2869866 APC-conjuagted-anti-CD11b BD Biosciences Cat#553312; RRID:AB_398535 APC-conjuagted-anti-CD206 Thermo Fisher Scientific Cat#17-2061-82; RRID:AB_2637420 APC-conjuagted-anti-GzmB Thermo Fisher Scientific Cat#17-8898-82; RRID:AB_2688068 PE-conjuagted-anti-CD86 Thermo Fisher Scientific Cat#12-0862-82; RRID:AB_465768 OVA257-264 (SIINFEKL) peptide bound to H-2Kb Monoclonal Antibody-APC Thermo Fisher Scientific Cat#17-5743-80; RRID:AB_1311288 FITC-conjuagted-anti-CD45.1 Biolegend Cat#110705; RRID:AB_313494 Anti-GSDMD rabbit mAb Abcam Cat#ab209845; RRID:AB_2783550 Anti-tubulin rabbit mAb ABclonal Cat#A12289; RRID:AB_2861647 HRP-conjugated Goat anti-Rabbit IgG ABclonal Cat#AS014; RRID:AB_2769854 Bacterial and virus strains Salmonella Typhimurium VNP20009 (VNP) ATCC Cat#202165 VNP-2 (VNP strains constitutively expresses RFP) This paper N/A DLV (VNP strains defective in lon gene) This paper N/A DLV-1(DLV strains constitutively expresses sfGFP) This paper N/A DLV-2 (DLV strains constitutively expresses RFP) This paper N/A DLV-3(DLV strains constitutively expresses luxCDABE) This paper N/A DLV-4(DLV strains constitutively expresses Nirfp) This paper N/A DLV-5(DLV strains expresses RFP with the aid of the sifB promoter) This paper N/A DLV-6(DLV strains expresses lysE with the aid of the sifB promoter) This paper N/A Chemicals, peptides, and recombinant proteins Mycoplasma removal reagent Yeasen Cat#40607ES03 transfection reagents Yeasen Cat#40802ES08 fixable viability dye BD Cat#564407 Hoechst dye KeyGEN BioTECH Cat#KGA1804-10 (Continued on next page) 18 Cell Reports 45, 116918, February 24, 2026 .. REAGENT or RESOURCE SOURCE IDENTIFIER Near-infrared lipophilic carbocyanine tracer DiR KeyGEN BioTECH Cat#KGE2603-5 Rhodamine MACKLIN Cat#R817228 ICG MACKLIN Cat#I953656 Luciferase substrate Vazyme Cat#DD1210 H2DCFDA MedChemExpress Cat#HY-D0940 Triton X-100 Sigma-Aldrich Cat#93443 M-CSF Yeasen Cat#91109ES10 GM-CSF Diane Biologics Cat#PDEM100050 IL4 protein Bioworld Cat#BK0246 DMF MedChemExpress Cat#HY-17363 DSF MedChemExpress Cat#HY-B0240 TAK-242 Selleck Cat#S7455 TH1020 MedChemExpress Cat#HY-116961 anti-PD1 antibody MedChemExpress Cat#A2122 Critical commercial assays Uniclone One-Step Seamless Cloning Kit Genesand Cat#SC612 T4 DNA ligase Sangon Biotech Cat#B600511-0001 FreeZol Reagent Vazyme Cat#R711-01 Reverse transcription kit Thermo Scientific Cat#M16315 AceQ qPCR SYBR Green Master Mix Vazyme Cat#Q221-01 permeabilization and fixation kit BD Biosciences Cat#555028 LDH Cytotoxicity Assay Kit Solarbio Cat#BC0680 ABplex Mouse Cytokine 15-Plex Assay Kit Abclonal Cat#RK05203 HMGB1 ELISA kit BYabscience Cat#BYHS504658 Deposited data Data files for RNA-sequencing This paper GEO: GSE291580 Experimental models: Cell lines 4T1 ATCC Cat#CRL-2539 H22 BLUEFBIO Cat#BFN608007276 A20 ATCC Cat#TIB-208 Panc02 Servicebio Cat Polyacrylamide Gel Electrophoresis:Article Title: Targeting CTGF overcomes resistance to CSF1R inhibitors by preventing CAF activation in colorectal cancer. Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER BLZ945 MCE Cat#HY-12768 Pamrevlumab (anti-CTGF) Selleck Cat#A2042 EDHB sigma Cat# E24859 LY294002 MCE Cat#HY-10108 740-Y-P MCE Cat#HY-P0175 Anti-PD-1 monoclonal antibody selleck Cat#A2122 Matrigel VivoMatter Biotech Cat#VM002-PRF-10 Organoid Complete Medium VivoMatter Biotech Cat#RSQ-OCM1102 Fetal Bovine Serum Umedium Cat#3021A Critical commercial assays Masson’s Trichrome Stain Kit Solarbio Cat#G1340 Sirius Red Stain Kit Solarbio Cat#G1472 Mouse CTGF ELISA KIT Shanghai Enzyme-linked Biotechnology Cat#ml037697 Human CTGF ELISA KIT Shanghai Enzyme-linked Biotechnology Cat#ml025961 Omni-Easy TM One-step Color PAGE Gel Rapid Preparation Kit EpiZyme Cat#PG212 Multiplex Fluorescent Immunohistochemistry Staining Kit absin Cat#abs50029 PrimeScript TM FAST RT reagent Kit with gDNA Eraser Takara Cat#RR092A Talent qPCR PreMix TIANGEN Cat#FP209 Deposited data Bulk RNA-seq raw data This paper GSA:PRJCA040243 IMC raw data This paper GSA:OMIX012148 Experimental models: Cell lines CT26 Pricella Biotechnology Cat#CL-0071 CMT93 Pricella Biotechnology Cat#CL-0920 NIH/3T3 Pricella Biotechnology Cat#CL-0171 HCT-8 Pricella Biotechnology Cat#CL-0098 Caco2 Pricella Biotechnology Cat#CL-0050 HCT15 Pricella Biotechnology Cat#CL-0097 HT29 Pricella Biotechnology Cat#CL-0118 HCT116 Pricella Biotechnology Cat# Multiplex Assay:Article Title: Targeting CTGF overcomes resistance to CSF1R inhibitors by preventing CAF activation in colorectal cancer. Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER BLZ945 MCE Cat#HY-12768 Pamrevlumab (anti-CTGF) Selleck Cat#A2042 EDHB sigma Cat# E24859 LY294002 MCE Cat#HY-10108 740-Y-P MCE Cat#HY-P0175 Anti-PD-1 monoclonal antibody selleck Cat#A2122 Matrigel VivoMatter Biotech Cat#VM002-PRF-10 Organoid Complete Medium VivoMatter Biotech Cat#RSQ-OCM1102 Fetal Bovine Serum Umedium Cat#3021A Critical commercial assays Masson’s Trichrome Stain Kit Solarbio Cat#G1340 Sirius Red Stain Kit Solarbio Cat#G1472 Mouse CTGF ELISA KIT Shanghai Enzyme-linked Biotechnology Cat#ml037697 Human CTGF ELISA KIT Shanghai Enzyme-linked Biotechnology Cat#ml025961 Omni-Easy TM One-step Color PAGE Gel Rapid Preparation Kit EpiZyme Cat#PG212 Multiplex Fluorescent Immunohistochemistry Staining Kit absin Cat#abs50029 PrimeScript TM FAST RT reagent Kit with gDNA Eraser Takara Cat#RR092A Talent qPCR PreMix TIANGEN Cat#FP209 Deposited data Bulk RNA-seq raw data This paper GSA:PRJCA040243 IMC raw data This paper GSA:OMIX012148 Experimental models: Cell lines CT26 Pricella Biotechnology Cat#CL-0071 CMT93 Pricella Biotechnology Cat#CL-0920 NIH/3T3 Pricella Biotechnology Cat#CL-0171 HCT-8 Pricella Biotechnology Cat#CL-0098 Caco2 Pricella Biotechnology Cat#CL-0050 HCT15 Pricella Biotechnology Cat#CL-0097 HT29 Pricella Biotechnology Cat#CL-0118 HCT116 Pricella Biotechnology Cat# RNA Sequencing:Article Title: Targeting CTGF overcomes resistance to CSF1R inhibitors by preventing CAF activation in colorectal cancer. Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER BLZ945 MCE Cat#HY-12768 Pamrevlumab (anti-CTGF) Selleck Cat#A2042 EDHB sigma Cat# E24859 LY294002 MCE Cat#HY-10108 740-Y-P MCE Cat#HY-P0175 Anti-PD-1 monoclonal antibody selleck Cat#A2122 Matrigel VivoMatter Biotech Cat#VM002-PRF-10 Organoid Complete Medium VivoMatter Biotech Cat#RSQ-OCM1102 Fetal Bovine Serum Umedium Cat#3021A Critical commercial assays Masson’s Trichrome Stain Kit Solarbio Cat#G1340 Sirius Red Stain Kit Solarbio Cat#G1472 Mouse CTGF ELISA KIT Shanghai Enzyme-linked Biotechnology Cat#ml037697 Human CTGF ELISA KIT Shanghai Enzyme-linked Biotechnology Cat#ml025961 Omni-Easy TM One-step Color PAGE Gel Rapid Preparation Kit EpiZyme Cat#PG212 Multiplex Fluorescent Immunohistochemistry Staining Kit absin Cat#abs50029 PrimeScript TM FAST RT reagent Kit with gDNA Eraser Takara Cat#RR092A Talent qPCR PreMix TIANGEN Cat#FP209 Deposited data Bulk RNA-seq raw data This paper GSA:PRJCA040243 IMC raw data This paper GSA:OMIX012148 Experimental models: Cell lines CT26 Pricella Biotechnology Cat#CL-0071 CMT93 Pricella Biotechnology Cat#CL-0920 NIH/3T3 Pricella Biotechnology Cat#CL-0171 HCT-8 Pricella Biotechnology Cat#CL-0098 Caco2 Pricella Biotechnology Cat#CL-0050 HCT15 Pricella Biotechnology Cat#CL-0097 HT29 Pricella Biotechnology Cat#CL-0118 HCT116 Pricella Biotechnology Cat# Virus:Article Title: β2AR as a target in methamphetamine addiction: Divergent mechanisms from TAAR1. Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies monoclonal anti-FLAG M2–fluorescein isothiocyanate antibody Sigma-Aldrich cat#F1804;RRID:AB_262044 AMPA Receptor 1 (GluA1) Antibody CST cat#13185;RRID:AB_2732897 AMPA Receptor 2 (GluA2) Antibody CST cat#13607;RRID:AB_2650557 AMPA Receptor 3 (GluA3) Antibody CST cat#4676;RRID:AB_10547136 Phospho-AMPA Receptor1 (GluA1)(Ser845) Antibody CST cat#8084;RRID:AB_10860773 Phospho-AMPA Receptor 1 (GluA1)(Ser831) Antibody CST cat#75574;RRID:AB_2799873 Phospho-AMPA Receptor 2 (GluA2) (Tyr869/Tyr873/Tyr876) Antibody CST cat#3921;RRID:AB_1642146 Mouse Anti-β actin mAb ZSGB-BIO cat#TA-09;RRID:AB_2636897 Bacterial and virus strains rAAV-D1-mCherry BrainVTA cat#PT-0757 rAAV-D2-EGFP BrainVTA cat#PT-3245 DH5α TIANGEN cat#CB101-03 Bac-to-Bac Baculovirus Expression System Invitrogen cat#10359016 Chemicals, peptides, and recombinant proteins S(+)-Methamphetamine (S-METH) Sigma-Aldrich-Supelco cat#M-020 R(− )-Methamphetamine (R-METH) Sigma-Aldrich-Supelco cat#M-024 (±)-MDMA Sigma-Aldrich-Supelco cat#M-013 (±)-Amphetamine Stanford Chemicals cat#AMP-0990-10 Epinephrine Sigma-Aldrich cat#E4250 Zenidolol hydrochloride MedChemExpress cat#HY-13951 Clenbuterol hydrochloride MedChemExpress cat#HY-B1614 RO 5212773 MedChemExpress cat#HY-110098 RO5166017 MedChemExpress cat#HY-12699 Coelenterazine 400a NanoLight cat#340-500 Lauryl Maltose Neopentyl Glycol (LMNG) Anatrace cat#NG310 Cholesterol Hemisuccinate TRIS Salt (CHS) Anatrace cat#CH210 Apyrase NEB cat#M0398S Critical commercial assays HTRF cAMP Gs Dynamic Detection Kit Cisbio cat#62a.m.4PEC HTRF IP-One Gq Detection Kit Cisbio cat#62IPAPEC Deposited data METH-β2AR-G s PDB protein bank (https://www.rcsb.org/) PDB: 9U9V METH-β2AR-G s The Electron Microscopy DataBank (https://www.ebi.ac.uk/emdb/) EMDB: EMD-63971 Experimental models: Cell lines Freestyle 293-F cells Invitrogen Cat#R790-07 Article Title: HUWE1 stimulates mTORC1 activity by enhancing Rheb interaction with mTORC1 and supports de novo pyrimidine synthesis. Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Mouse monoclonal anti-HA (clone 16B12) Covance Cat#MMS-101R; RRID:AB_291262 Mouse monoclonal anti-ubiquitinylated proteins (FK2) MilliporeSigma Cat#04–263; RRID:AB_612093 Alexa Fluor 594-goat polyclonal anti-rabbit IgG Thermo Fisher Scientific Cat#A11012; RRID:AB_141359 Alexa Fluor 488-goat polyclonal anti-mouse IgG Thermo Fisher Scientific Cat#A11029; RRID:AB_138404 Alexa Fluor 594-goat polyclonal anti-mouse IgG Thermo Fisher Scientific Cat#R37121; RRID:AB_2556549 Anti-rabbit IgG HRP-Linked Whole Ab GE Healthcare Cat#NA934; RRID:AB_772206 Anti-mouse IgG HRP-Linked Whole Ab GE Healthcare Cat#NA931; RRID:AB_772210 Bacterial and virus strains One Shot TM Stbl TM Chemically Competent E. coli Thermo Fisher Scientific Cat#C737303 Chemicals, peptides, and recombinant proteins Amino acid mixtures Sigma-Aldrich Cat#R7131 L-Glutathione reduced Sigma-Aldrich Cat#G4251 N-Ethylmaleimide (NEM) Sigma-Aldrich Cat#E3876 Saponin Sigma-Aldrich Cat#S4521 Actinomycin D Sigma-Aldrich Cat#A9415 Lipofectamine 3000 Invitrogen Cat#L3000001 Amino acid-free DMEM medium US Biological Life Sciences Cat#D9800-27 Leucine-free DMEM medium Crystalgen Cat#226-024 L-leucine RPI Cat#L22000 Dialyzed Fetal Bovine Serum Thermo Fisher Scientific Cat#26400044 Pierce TM Glutathione Agarose Thermo Fisher Scientific Cat#16100 Prolong Gold antifade reagent with DAPI Thermo Fisher Scientific Cat#P36931 Protein G Sepharose 4 Fast Flow GE Healthcare Cat#17061801 Flag peptide LifeTein Cat#LT12022 Insulin Sigma-Aldrich Cat#10908 Phosphorus-32 NEN Radiochemicals Cat#NEX011 MgATP Solution R&D Systems Cat#B-20 Critical commercial assays Subcellular Protein Fractionation Kit Thermo Fisher Scientific Cat#78840 TriFECTa RNAi Kits Integrated DNA Technologies (IDT) N/A Experimental models: Cell lines Human: Electron Microscopy:Article Title: β2AR as a target in methamphetamine addiction: Divergent mechanisms from TAAR1. Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies monoclonal anti-FLAG M2–fluorescein isothiocyanate antibody Sigma-Aldrich cat#F1804;RRID:AB_262044 AMPA Receptor 1 (GluA1) Antibody CST cat#13185;RRID:AB_2732897 AMPA Receptor 2 (GluA2) Antibody CST cat#13607;RRID:AB_2650557 AMPA Receptor 3 (GluA3) Antibody CST cat#4676;RRID:AB_10547136 Phospho-AMPA Receptor1 (GluA1)(Ser845) Antibody CST cat#8084;RRID:AB_10860773 Phospho-AMPA Receptor 1 (GluA1)(Ser831) Antibody CST cat#75574;RRID:AB_2799873 Phospho-AMPA Receptor 2 (GluA2) (Tyr869/Tyr873/Tyr876) Antibody CST cat#3921;RRID:AB_1642146 Mouse Anti-β actin mAb ZSGB-BIO cat#TA-09;RRID:AB_2636897 Bacterial and virus strains rAAV-D1-mCherry BrainVTA cat#PT-0757 rAAV-D2-EGFP BrainVTA cat#PT-3245 DH5α TIANGEN cat#CB101-03 Bac-to-Bac Baculovirus Expression System Invitrogen cat#10359016 Chemicals, peptides, and recombinant proteins S(+)-Methamphetamine (S-METH) Sigma-Aldrich-Supelco cat#M-020 R(− )-Methamphetamine (R-METH) Sigma-Aldrich-Supelco cat#M-024 (±)-MDMA Sigma-Aldrich-Supelco cat#M-013 (±)-Amphetamine Stanford Chemicals cat#AMP-0990-10 Epinephrine Sigma-Aldrich cat#E4250 Zenidolol hydrochloride MedChemExpress cat#HY-13951 Clenbuterol hydrochloride MedChemExpress cat#HY-B1614 RO 5212773 MedChemExpress cat#HY-110098 RO5166017 MedChemExpress cat#HY-12699 Coelenterazine 400a NanoLight cat#340-500 Lauryl Maltose Neopentyl Glycol (LMNG) Anatrace cat#NG310 Cholesterol Hemisuccinate TRIS Salt (CHS) Anatrace cat#CH210 Apyrase NEB cat#M0398S Critical commercial assays HTRF cAMP Gs Dynamic Detection Kit Cisbio cat#62a.m.4PEC HTRF IP-One Gq Detection Kit Cisbio cat#62IPAPEC Deposited data METH-β2AR-G s PDB protein bank (https://www.rcsb.org/) PDB: 9U9V METH-β2AR-G s The Electron Microscopy DataBank (https://www.ebi.ac.uk/emdb/) EMDB: EMD-63971 Experimental models: Cell lines Freestyle 293-F cells Invitrogen Cat#R790-07 Plasmid Preparation:Article Title: Optogenetic manipulation of estrogen receptor signaling to improve estrogen deficiency Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies anti-GAPDH Abcam Cat#ab181603; RRID: AB_2687666 anti-pS2 Abcam Cat#ab92377; RRID:AB_10562112 anti-Ki67 CST Cat#12202; RRID:AB_2620142 anti-P53 Proteintech Cat#10442-1-AP; RRID:AB_2206609 anti-ERα Santa Cruz Cat#sc-8002; RRID:AB_627558 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) Abcam Cat#ab150113; RRID:AB_2576208 Goat Anti-Rabbit IgG-HRP Abmart Cat#M21002; RRID:AB_2713951 Chemicals, peptides, and recombinant proteins DMEM GIBCO Cat#C11995500BT penicillin/streptomycin solution Beyotime Cat#C0222 phenol red-free DMEM Cienry Cat#CR-12900 charcoal-depleted FBS BI Cat#04-201-1A FBS Sigma Cat#F8318 17β-Estradiol Sigma-Aldrich Cat#E8875 TRIzol Invitrogen Cat#15596018 1×SDS-PAGE sample loading buffer Beyotime Cat#P0015A cOmplete TM Roche Cat#4693132001 (Hydroxypropyl)methyl cellulose Sigma-Aldrich Cat#423173 Pluronic F127 Sigma-Aldrich Cat#P2443 Schiff’s reagent Haokebio Cat#HK2022 OCT embedding agent Servicebio Cat#G6059 Universal tissue fixative Servicebio Cat#G1101 Critical commercial assays Lipofectamine 3000 Thermo Cat# L3000015 Fast All-in-One RT Kit ES Science Cat#RT001 2×Super SYBR Green qPCR Master Mix ES Science Cat#QP002 CCK8 Beyotime Cat#C0037 Deposited data high-affinity estrogen response elements in human Bourdeau et al. 34 http://mapageweb.umontreal.ca/maders/ eredatabase/#:∼:text=We%20have %20screened%20for%20high%20affinity %20EREs%20%28perfect,∼70% 2C000%20motifs%20in%20the%20human %20and%20mouse%20genomes. .. Experimental models: Cell lines MCF-7 ATCC Cat#HTB-22 Fractionation:Article Title: HUWE1 stimulates mTORC1 activity by enhancing Rheb interaction with mTORC1 and supports de novo pyrimidine synthesis. Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Mouse monoclonal anti-HA (clone 16B12) Covance Cat#MMS-101R; RRID:AB_291262 Mouse monoclonal anti-ubiquitinylated proteins (FK2) MilliporeSigma Cat#04–263; RRID:AB_612093 Alexa Fluor 594-goat polyclonal anti-rabbit IgG Thermo Fisher Scientific Cat#A11012; RRID:AB_141359 Alexa Fluor 488-goat polyclonal anti-mouse IgG Thermo Fisher Scientific Cat#A11029; RRID:AB_138404 Alexa Fluor 594-goat polyclonal anti-mouse IgG Thermo Fisher Scientific Cat#R37121; RRID:AB_2556549 Anti-rabbit IgG HRP-Linked Whole Ab GE Healthcare Cat#NA934; RRID:AB_772206 Anti-mouse IgG HRP-Linked Whole Ab GE Healthcare Cat#NA931; RRID:AB_772210 Bacterial and virus strains One Shot TM Stbl TM Chemically Competent E. coli Thermo Fisher Scientific Cat#C737303 Chemicals, peptides, and recombinant proteins Amino acid mixtures Sigma-Aldrich Cat#R7131 L-Glutathione reduced Sigma-Aldrich Cat#G4251 N-Ethylmaleimide (NEM) Sigma-Aldrich Cat#E3876 Saponin Sigma-Aldrich Cat#S4521 Actinomycin D Sigma-Aldrich Cat#A9415 Lipofectamine 3000 Invitrogen Cat#L3000001 Amino acid-free DMEM medium US Biological Life Sciences Cat#D9800-27 Leucine-free DMEM medium Crystalgen Cat#226-024 L-leucine RPI Cat#L22000 Dialyzed Fetal Bovine Serum Thermo Fisher Scientific Cat#26400044 Pierce TM Glutathione Agarose Thermo Fisher Scientific Cat#16100 Prolong Gold antifade reagent with DAPI Thermo Fisher Scientific Cat#P36931 Protein G Sepharose 4 Fast Flow GE Healthcare Cat#17061801 Flag peptide LifeTein Cat#LT12022 Insulin Sigma-Aldrich Cat#10908 Phosphorus-32 NEN Radiochemicals Cat#NEX011 MgATP Solution R&D Systems Cat#B-20 Critical commercial assays Subcellular Protein Fractionation Kit Thermo Fisher Scientific Cat#78840 TriFECTa RNAi Kits Integrated DNA Technologies (IDT) N/A Experimental models: Cell lines Human: shRNA:Article Title: HUWE1 stimulates mTORC1 activity by enhancing Rheb interaction with mTORC1 and supports de novo pyrimidine synthesis. Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Mouse monoclonal anti-HA (clone 16B12) Covance Cat#MMS-101R; RRID:AB_291262 Mouse monoclonal anti-ubiquitinylated proteins (FK2) MilliporeSigma Cat#04–263; RRID:AB_612093 Alexa Fluor 594-goat polyclonal anti-rabbit IgG Thermo Fisher Scientific Cat#A11012; RRID:AB_141359 Alexa Fluor 488-goat polyclonal anti-mouse IgG Thermo Fisher Scientific Cat#A11029; RRID:AB_138404 Alexa Fluor 594-goat polyclonal anti-mouse IgG Thermo Fisher Scientific Cat#R37121; RRID:AB_2556549 Anti-rabbit IgG HRP-Linked Whole Ab GE Healthcare Cat#NA934; RRID:AB_772206 Anti-mouse IgG HRP-Linked Whole Ab GE Healthcare Cat#NA931; RRID:AB_772210 Bacterial and virus strains One Shot TM Stbl TM Chemically Competent E. coli Thermo Fisher Scientific Cat#C737303 Chemicals, peptides, and recombinant proteins Amino acid mixtures Sigma-Aldrich Cat#R7131 L-Glutathione reduced Sigma-Aldrich Cat#G4251 N-Ethylmaleimide (NEM) Sigma-Aldrich Cat#E3876 Saponin Sigma-Aldrich Cat#S4521 Actinomycin D Sigma-Aldrich Cat#A9415 Lipofectamine 3000 Invitrogen Cat#L3000001 Amino acid-free DMEM medium US Biological Life Sciences Cat#D9800-27 Leucine-free DMEM medium Crystalgen Cat#226-024 L-leucine RPI Cat#L22000 Dialyzed Fetal Bovine Serum Thermo Fisher Scientific Cat#26400044 Pierce TM Glutathione Agarose Thermo Fisher Scientific Cat#16100 Prolong Gold antifade reagent with DAPI Thermo Fisher Scientific Cat#P36931 Protein G Sepharose 4 Fast Flow GE Healthcare Cat#17061801 Flag peptide LifeTein Cat#LT12022 Insulin Sigma-Aldrich Cat#10908 Phosphorus-32 NEN Radiochemicals Cat#NEX011 MgATP Solution R&D Systems Cat#B-20 Critical commercial assays Subcellular Protein Fractionation Kit Thermo Fisher Scientific Cat#78840 TriFECTa RNAi Kits Integrated DNA Technologies (IDT) N/A Experimental models: Cell lines Human: Luciferase:Article Title: Controlled pyroptosis of engineered macrophages enables biphasic antitumor via the release of oncolytic bacteria and inflammatory signals. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies FITC-conjuagted-anti-MHCII BD Biosciences Cat#562009; RRID:AB_10893593 FITC-conjuagted-anti-CD3 BD Biosciences Cat#561801; RRID:AB_10895388 FITC-conjuagted-anti-CD11b BD Biosciences Cat#561684; RRID:AB_10893796 FITC-conjuagted-anti-CD44 BD Biosciences Cat#553133; RRID:AB_2076224 PE-Cy7-conjuagted-anti-CD11c BD Biosciences Cat#558079; RRID:AB_647251 PE-Cy7-conjuagted-anti-CD8 BD Biosciences Cat#552877; RRID:AB_394506 PE-conjuagted-anti-CD8 BD Biosciences Cat#554857; RRID:AB_395558 PE-conjuagted-anti-Foxp3 BD Biosciences Cat#563101; RRID:AB_2738006 PerCP-Cy5.5-conjuagted-anti-CD4 BD Biosciences Cat#550954; RRID:AB_393977 BV421-conjuagted-anti-F4/80 BD Biosciences Cat#565411; RRID:AB_2734779 BV510-conjuagted-anti-CD45 BD Biosciences Cat#740131; RRID:AB_2739888 APC-conjuagted-anti-CD62L BD Biosciences Cat#553152; RRID:AB_398533 APC-conjuagted-anti-F4/80 BD Biosciences Cat#566787; RRID:AB_2869866 APC-conjuagted-anti-CD11b BD Biosciences Cat#553312; RRID:AB_398535 APC-conjuagted-anti-CD206 Thermo Fisher Scientific Cat#17-2061-82; RRID:AB_2637420 APC-conjuagted-anti-GzmB Thermo Fisher Scientific Cat#17-8898-82; RRID:AB_2688068 PE-conjuagted-anti-CD86 Thermo Fisher Scientific Cat#12-0862-82; RRID:AB_465768 OVA257-264 (SIINFEKL) peptide bound to H-2Kb Monoclonal Antibody-APC Thermo Fisher Scientific Cat#17-5743-80; RRID:AB_1311288 FITC-conjuagted-anti-CD45.1 Biolegend Cat#110705; RRID:AB_313494 Anti-GSDMD rabbit mAb Abcam Cat#ab209845; RRID:AB_2783550 Anti-tubulin rabbit mAb ABclonal Cat#A12289; RRID:AB_2861647 HRP-conjugated Goat anti-Rabbit IgG ABclonal Cat#AS014; RRID:AB_2769854 Bacterial and virus strains Salmonella Typhimurium VNP20009 (VNP) ATCC Cat#202165 VNP-2 (VNP strains constitutively expresses RFP) This paper N/A DLV (VNP strains defective in lon gene) This paper N/A DLV-1(DLV strains constitutively expresses sfGFP) This paper N/A DLV-2 (DLV strains constitutively expresses RFP) This paper N/A DLV-3(DLV strains constitutively expresses luxCDABE) This paper N/A DLV-4(DLV strains constitutively expresses Nirfp) This paper N/A DLV-5(DLV strains expresses RFP with the aid of the sifB promoter) This paper N/A DLV-6(DLV strains expresses lysE with the aid of the sifB promoter) This paper N/A Chemicals, peptides, and recombinant proteins Mycoplasma removal reagent Yeasen Cat#40607ES03 transfection reagents Yeasen Cat#40802ES08 fixable viability dye BD Cat#564407 Hoechst dye KeyGEN BioTECH Cat#KGA1804-10 (Continued on next page) 18 Cell Reports 45, 116918, February 24, 2026 .. REAGENT or RESOURCE SOURCE IDENTIFIER Near-infrared lipophilic carbocyanine tracer DiR KeyGEN BioTECH Cat#KGE2603-5 Rhodamine MACKLIN Cat#R817228 ICG MACKLIN Cat#I953656 Luciferase substrate Vazyme Cat#DD1210 H2DCFDA MedChemExpress Cat#HY-D0940 Triton X-100 Sigma-Aldrich Cat#93443 M-CSF Yeasen Cat#91109ES10 GM-CSF Diane Biologics Cat#PDEM100050 IL4 protein Bioworld Cat#BK0246 DMF MedChemExpress Cat#HY-17363 DSF MedChemExpress Cat#HY-B0240 TAK-242 Selleck Cat#S7455 TH1020 MedChemExpress Cat#HY-116961 anti-PD1 antibody MedChemExpress Cat#A2122 Critical commercial assays Uniclone One-Step Seamless Cloning Kit Genesand Cat#SC612 T4 DNA ligase Sangon Biotech Cat#B600511-0001 FreeZol Reagent Vazyme Cat#R711-01 Reverse transcription kit Thermo Scientific Cat#M16315 AceQ qPCR SYBR Green Master Mix Vazyme Cat#Q221-01 permeabilization and fixation kit BD Biosciences Cat#555028 LDH Cytotoxicity Assay Kit Solarbio Cat#BC0680 ABplex Mouse Cytokine 15-Plex Assay Kit Abclonal Cat#RK05203 HMGB1 ELISA kit BYabscience Cat#BYHS504658 Deposited data Data files for RNA-sequencing This paper GEO: GSE291580 Experimental models: Cell lines 4T1 ATCC Cat#CRL-2539 H22 BLUEFBIO Cat#BFN608007276 A20 ATCC Cat#TIB-208 Panc02 Servicebio Cat Cloning:Article Title: Controlled pyroptosis of engineered macrophages enables biphasic antitumor via the release of oncolytic bacteria and inflammatory signals. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies FITC-conjuagted-anti-MHCII BD Biosciences Cat#562009; RRID:AB_10893593 FITC-conjuagted-anti-CD3 BD Biosciences Cat#561801; RRID:AB_10895388 FITC-conjuagted-anti-CD11b BD Biosciences Cat#561684; RRID:AB_10893796 FITC-conjuagted-anti-CD44 BD Biosciences Cat#553133; RRID:AB_2076224 PE-Cy7-conjuagted-anti-CD11c BD Biosciences Cat#558079; RRID:AB_647251 PE-Cy7-conjuagted-anti-CD8 BD Biosciences Cat#552877; RRID:AB_394506 PE-conjuagted-anti-CD8 BD Biosciences Cat#554857; RRID:AB_395558 PE-conjuagted-anti-Foxp3 BD Biosciences Cat#563101; RRID:AB_2738006 PerCP-Cy5.5-conjuagted-anti-CD4 BD Biosciences Cat#550954; RRID:AB_393977 BV421-conjuagted-anti-F4/80 BD Biosciences Cat#565411; RRID:AB_2734779 BV510-conjuagted-anti-CD45 BD Biosciences Cat#740131; RRID:AB_2739888 APC-conjuagted-anti-CD62L BD Biosciences Cat#553152; RRID:AB_398533 APC-conjuagted-anti-F4/80 BD Biosciences Cat#566787; RRID:AB_2869866 APC-conjuagted-anti-CD11b BD Biosciences Cat#553312; RRID:AB_398535 APC-conjuagted-anti-CD206 Thermo Fisher Scientific Cat#17-2061-82; RRID:AB_2637420 APC-conjuagted-anti-GzmB Thermo Fisher Scientific Cat#17-8898-82; RRID:AB_2688068 PE-conjuagted-anti-CD86 Thermo Fisher Scientific Cat#12-0862-82; RRID:AB_465768 OVA257-264 (SIINFEKL) peptide bound to H-2Kb Monoclonal Antibody-APC Thermo Fisher Scientific Cat#17-5743-80; RRID:AB_1311288 FITC-conjuagted-anti-CD45.1 Biolegend Cat#110705; RRID:AB_313494 Anti-GSDMD rabbit mAb Abcam Cat#ab209845; RRID:AB_2783550 Anti-tubulin rabbit mAb ABclonal Cat#A12289; RRID:AB_2861647 HRP-conjugated Goat anti-Rabbit IgG ABclonal Cat#AS014; RRID:AB_2769854 Bacterial and virus strains Salmonella Typhimurium VNP20009 (VNP) ATCC Cat#202165 VNP-2 (VNP strains constitutively expresses RFP) This paper N/A DLV (VNP strains defective in lon gene) This paper N/A DLV-1(DLV strains constitutively expresses sfGFP) This paper N/A DLV-2 (DLV strains constitutively expresses RFP) This paper N/A DLV-3(DLV strains constitutively expresses luxCDABE) This paper N/A DLV-4(DLV strains constitutively expresses Nirfp) This paper N/A DLV-5(DLV strains expresses RFP with the aid of the sifB promoter) This paper N/A DLV-6(DLV strains expresses lysE with the aid of the sifB promoter) This paper N/A Chemicals, peptides, and recombinant proteins Mycoplasma removal reagent Yeasen Cat#40607ES03 transfection reagents Yeasen Cat#40802ES08 fixable viability dye BD Cat#564407 Hoechst dye KeyGEN BioTECH Cat#KGA1804-10 (Continued on next page) 18 Cell Reports 45, 116918, February 24, 2026 .. REAGENT or RESOURCE SOURCE IDENTIFIER Near-infrared lipophilic carbocyanine tracer DiR KeyGEN BioTECH Cat#KGE2603-5 Rhodamine MACKLIN Cat#R817228 ICG MACKLIN Cat#I953656 Luciferase substrate Vazyme Cat#DD1210 H2DCFDA MedChemExpress Cat#HY-D0940 Triton X-100 Sigma-Aldrich Cat#93443 M-CSF Yeasen Cat#91109ES10 GM-CSF Diane Biologics Cat#PDEM100050 IL4 protein Bioworld Cat#BK0246 DMF MedChemExpress Cat#HY-17363 DSF MedChemExpress Cat#HY-B0240 TAK-242 Selleck Cat#S7455 TH1020 MedChemExpress Cat#HY-116961 anti-PD1 antibody MedChemExpress Cat#A2122 Critical commercial assays Uniclone One-Step Seamless Cloning Kit Genesand Cat#SC612 T4 DNA ligase Sangon Biotech Cat#B600511-0001 FreeZol Reagent Vazyme Cat#R711-01 Reverse transcription kit Thermo Scientific Cat#M16315 AceQ qPCR SYBR Green Master Mix Vazyme Cat#Q221-01 permeabilization and fixation kit BD Biosciences Cat#555028 LDH Cytotoxicity Assay Kit Solarbio Cat#BC0680 ABplex Mouse Cytokine 15-Plex Assay Kit Abclonal Cat#RK05203 HMGB1 ELISA kit BYabscience Cat#BYHS504658 Deposited data Data files for RNA-sequencing This paper GEO: GSE291580 Experimental models: Cell lines 4T1 ATCC Cat#CRL-2539 H22 BLUEFBIO Cat#BFN608007276 A20 ATCC Cat#TIB-208 Panc02 Servicebio Cat Reverse Transcription:Article Title: Controlled pyroptosis of engineered macrophages enables biphasic antitumor via the release of oncolytic bacteria and inflammatory signals. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies FITC-conjuagted-anti-MHCII BD Biosciences Cat#562009; RRID:AB_10893593 FITC-conjuagted-anti-CD3 BD Biosciences Cat#561801; RRID:AB_10895388 FITC-conjuagted-anti-CD11b BD Biosciences Cat#561684; RRID:AB_10893796 FITC-conjuagted-anti-CD44 BD Biosciences Cat#553133; RRID:AB_2076224 PE-Cy7-conjuagted-anti-CD11c BD Biosciences Cat#558079; RRID:AB_647251 PE-Cy7-conjuagted-anti-CD8 BD Biosciences Cat#552877; RRID:AB_394506 PE-conjuagted-anti-CD8 BD Biosciences Cat#554857; RRID:AB_395558 PE-conjuagted-anti-Foxp3 BD Biosciences Cat#563101; RRID:AB_2738006 PerCP-Cy5.5-conjuagted-anti-CD4 BD Biosciences Cat#550954; RRID:AB_393977 BV421-conjuagted-anti-F4/80 BD Biosciences Cat#565411; RRID:AB_2734779 BV510-conjuagted-anti-CD45 BD Biosciences Cat#740131; RRID:AB_2739888 APC-conjuagted-anti-CD62L BD Biosciences Cat#553152; RRID:AB_398533 APC-conjuagted-anti-F4/80 BD Biosciences Cat#566787; RRID:AB_2869866 APC-conjuagted-anti-CD11b BD Biosciences Cat#553312; RRID:AB_398535 APC-conjuagted-anti-CD206 Thermo Fisher Scientific Cat#17-2061-82; RRID:AB_2637420 APC-conjuagted-anti-GzmB Thermo Fisher Scientific Cat#17-8898-82; RRID:AB_2688068 PE-conjuagted-anti-CD86 Thermo Fisher Scientific Cat#12-0862-82; RRID:AB_465768 OVA257-264 (SIINFEKL) peptide bound to H-2Kb Monoclonal Antibody-APC Thermo Fisher Scientific Cat#17-5743-80; RRID:AB_1311288 FITC-conjuagted-anti-CD45.1 Biolegend Cat#110705; RRID:AB_313494 Anti-GSDMD rabbit mAb Abcam Cat#ab209845; RRID:AB_2783550 Anti-tubulin rabbit mAb ABclonal Cat#A12289; RRID:AB_2861647 HRP-conjugated Goat anti-Rabbit IgG ABclonal Cat#AS014; RRID:AB_2769854 Bacterial and virus strains Salmonella Typhimurium VNP20009 (VNP) ATCC Cat#202165 VNP-2 (VNP strains constitutively expresses RFP) This paper N/A DLV (VNP strains defective in lon gene) This paper N/A DLV-1(DLV strains constitutively expresses sfGFP) This paper N/A DLV-2 (DLV strains constitutively expresses RFP) This paper N/A DLV-3(DLV strains constitutively expresses luxCDABE) This paper N/A DLV-4(DLV strains constitutively expresses Nirfp) This paper N/A DLV-5(DLV strains expresses RFP with the aid of the sifB promoter) This paper N/A DLV-6(DLV strains expresses lysE with the aid of the sifB promoter) This paper N/A Chemicals, peptides, and recombinant proteins Mycoplasma removal reagent Yeasen Cat#40607ES03 transfection reagents Yeasen Cat#40802ES08 fixable viability dye BD Cat#564407 Hoechst dye KeyGEN BioTECH Cat#KGA1804-10 (Continued on next page) 18 Cell Reports 45, 116918, February 24, 2026 .. REAGENT or RESOURCE SOURCE IDENTIFIER Near-infrared lipophilic carbocyanine tracer DiR KeyGEN BioTECH Cat#KGE2603-5 Rhodamine MACKLIN Cat#R817228 ICG MACKLIN Cat#I953656 Luciferase substrate Vazyme Cat#DD1210 H2DCFDA MedChemExpress Cat#HY-D0940 Triton X-100 Sigma-Aldrich Cat#93443 M-CSF Yeasen Cat#91109ES10 GM-CSF Diane Biologics Cat#PDEM100050 IL4 protein Bioworld Cat#BK0246 DMF MedChemExpress Cat#HY-17363 DSF MedChemExpress Cat#HY-B0240 TAK-242 Selleck Cat#S7455 TH1020 MedChemExpress Cat#HY-116961 anti-PD1 antibody MedChemExpress Cat#A2122 Critical commercial assays Uniclone One-Step Seamless Cloning Kit Genesand Cat#SC612 T4 DNA ligase Sangon Biotech Cat#B600511-0001 FreeZol Reagent Vazyme Cat#R711-01 Reverse transcription kit Thermo Scientific Cat#M16315 AceQ qPCR SYBR Green Master Mix Vazyme Cat#Q221-01 permeabilization and fixation kit BD Biosciences Cat#555028 LDH Cytotoxicity Assay Kit Solarbio Cat#BC0680 ABplex Mouse Cytokine 15-Plex Assay Kit Abclonal Cat#RK05203 HMGB1 ELISA kit BYabscience Cat#BYHS504658 Deposited data Data files for RNA-sequencing This paper GEO: GSE291580 Experimental models: Cell lines 4T1 ATCC Cat#CRL-2539 H22 BLUEFBIO Cat#BFN608007276 A20 ATCC Cat#TIB-208 Panc02 Servicebio Cat SYBR Green Assay:Article Title: Controlled pyroptosis of engineered macrophages enables biphasic antitumor via the release of oncolytic bacteria and inflammatory signals. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies FITC-conjuagted-anti-MHCII BD Biosciences Cat#562009; RRID:AB_10893593 FITC-conjuagted-anti-CD3 BD Biosciences Cat#561801; RRID:AB_10895388 FITC-conjuagted-anti-CD11b BD Biosciences Cat#561684; RRID:AB_10893796 FITC-conjuagted-anti-CD44 BD Biosciences Cat#553133; RRID:AB_2076224 PE-Cy7-conjuagted-anti-CD11c BD Biosciences Cat#558079; RRID:AB_647251 PE-Cy7-conjuagted-anti-CD8 BD Biosciences Cat#552877; RRID:AB_394506 PE-conjuagted-anti-CD8 BD Biosciences Cat#554857; RRID:AB_395558 PE-conjuagted-anti-Foxp3 BD Biosciences Cat#563101; RRID:AB_2738006 PerCP-Cy5.5-conjuagted-anti-CD4 BD Biosciences Cat#550954; RRID:AB_393977 BV421-conjuagted-anti-F4/80 BD Biosciences Cat#565411; RRID:AB_2734779 BV510-conjuagted-anti-CD45 BD Biosciences Cat#740131; RRID:AB_2739888 APC-conjuagted-anti-CD62L BD Biosciences Cat#553152; RRID:AB_398533 APC-conjuagted-anti-F4/80 BD Biosciences Cat#566787; RRID:AB_2869866 APC-conjuagted-anti-CD11b BD Biosciences Cat#553312; RRID:AB_398535 APC-conjuagted-anti-CD206 Thermo Fisher Scientific Cat#17-2061-82; RRID:AB_2637420 APC-conjuagted-anti-GzmB Thermo Fisher Scientific Cat#17-8898-82; RRID:AB_2688068 PE-conjuagted-anti-CD86 Thermo Fisher Scientific Cat#12-0862-82; RRID:AB_465768 OVA257-264 (SIINFEKL) peptide bound to H-2Kb Monoclonal Antibody-APC Thermo Fisher Scientific Cat#17-5743-80; RRID:AB_1311288 FITC-conjuagted-anti-CD45.1 Biolegend Cat#110705; RRID:AB_313494 Anti-GSDMD rabbit mAb Abcam Cat#ab209845; RRID:AB_2783550 Anti-tubulin rabbit mAb ABclonal Cat#A12289; RRID:AB_2861647 HRP-conjugated Goat anti-Rabbit IgG ABclonal Cat#AS014; RRID:AB_2769854 Bacterial and virus strains Salmonella Typhimurium VNP20009 (VNP) ATCC Cat#202165 VNP-2 (VNP strains constitutively expresses RFP) This paper N/A DLV (VNP strains defective in lon gene) This paper N/A DLV-1(DLV strains constitutively expresses sfGFP) This paper N/A DLV-2 (DLV strains constitutively expresses RFP) This paper N/A DLV-3(DLV strains constitutively expresses luxCDABE) This paper N/A DLV-4(DLV strains constitutively expresses Nirfp) This paper N/A DLV-5(DLV strains expresses RFP with the aid of the sifB promoter) This paper N/A DLV-6(DLV strains expresses lysE with the aid of the sifB promoter) This paper N/A Chemicals, peptides, and recombinant proteins Mycoplasma removal reagent Yeasen Cat#40607ES03 transfection reagents Yeasen Cat#40802ES08 fixable viability dye BD Cat#564407 Hoechst dye KeyGEN BioTECH Cat#KGA1804-10 (Continued on next page) 18 Cell Reports 45, 116918, February 24, 2026 .. REAGENT or RESOURCE SOURCE IDENTIFIER Near-infrared lipophilic carbocyanine tracer DiR KeyGEN BioTECH Cat#KGE2603-5 Rhodamine MACKLIN Cat#R817228 ICG MACKLIN Cat#I953656 Luciferase substrate Vazyme Cat#DD1210 H2DCFDA MedChemExpress Cat#HY-D0940 Triton X-100 Sigma-Aldrich Cat#93443 M-CSF Yeasen Cat#91109ES10 GM-CSF Diane Biologics Cat#PDEM100050 IL4 protein Bioworld Cat#BK0246 DMF MedChemExpress Cat#HY-17363 DSF MedChemExpress Cat#HY-B0240 TAK-242 Selleck Cat#S7455 TH1020 MedChemExpress Cat#HY-116961 anti-PD1 antibody MedChemExpress Cat#A2122 Critical commercial assays Uniclone One-Step Seamless Cloning Kit Genesand Cat#SC612 T4 DNA ligase Sangon Biotech Cat#B600511-0001 FreeZol Reagent Vazyme Cat#R711-01 Reverse transcription kit Thermo Scientific Cat#M16315 AceQ qPCR SYBR Green Master Mix Vazyme Cat#Q221-01 permeabilization and fixation kit BD Biosciences Cat#555028 LDH Cytotoxicity Assay Kit Solarbio Cat#BC0680 ABplex Mouse Cytokine 15-Plex Assay Kit Abclonal Cat#RK05203 HMGB1 ELISA kit BYabscience Cat#BYHS504658 Deposited data Data files for RNA-sequencing This paper GEO: GSE291580 Experimental models: Cell lines 4T1 ATCC Cat#CRL-2539 H22 BLUEFBIO Cat#BFN608007276 A20 ATCC Cat#TIB-208 Panc02 Servicebio Cat LDH Cytotoxicity Assay:Article Title: Controlled pyroptosis of engineered macrophages enables biphasic antitumor via the release of oncolytic bacteria and inflammatory signals. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies FITC-conjuagted-anti-MHCII BD Biosciences Cat#562009; RRID:AB_10893593 FITC-conjuagted-anti-CD3 BD Biosciences Cat#561801; RRID:AB_10895388 FITC-conjuagted-anti-CD11b BD Biosciences Cat#561684; RRID:AB_10893796 FITC-conjuagted-anti-CD44 BD Biosciences Cat#553133; RRID:AB_2076224 PE-Cy7-conjuagted-anti-CD11c BD Biosciences Cat#558079; RRID:AB_647251 PE-Cy7-conjuagted-anti-CD8 BD Biosciences Cat#552877; RRID:AB_394506 PE-conjuagted-anti-CD8 BD Biosciences Cat#554857; RRID:AB_395558 PE-conjuagted-anti-Foxp3 BD Biosciences Cat#563101; RRID:AB_2738006 PerCP-Cy5.5-conjuagted-anti-CD4 BD Biosciences Cat#550954; RRID:AB_393977 BV421-conjuagted-anti-F4/80 BD Biosciences Cat#565411; RRID:AB_2734779 BV510-conjuagted-anti-CD45 BD Biosciences Cat#740131; RRID:AB_2739888 APC-conjuagted-anti-CD62L BD Biosciences Cat#553152; RRID:AB_398533 APC-conjuagted-anti-F4/80 BD Biosciences Cat#566787; RRID:AB_2869866 APC-conjuagted-anti-CD11b BD Biosciences Cat#553312; RRID:AB_398535 APC-conjuagted-anti-CD206 Thermo Fisher Scientific Cat#17-2061-82; RRID:AB_2637420 APC-conjuagted-anti-GzmB Thermo Fisher Scientific Cat#17-8898-82; RRID:AB_2688068 PE-conjuagted-anti-CD86 Thermo Fisher Scientific Cat#12-0862-82; RRID:AB_465768 OVA257-264 (SIINFEKL) peptide bound to H-2Kb Monoclonal Antibody-APC Thermo Fisher Scientific Cat#17-5743-80; RRID:AB_1311288 FITC-conjuagted-anti-CD45.1 Biolegend Cat#110705; RRID:AB_313494 Anti-GSDMD rabbit mAb Abcam Cat#ab209845; RRID:AB_2783550 Anti-tubulin rabbit mAb ABclonal Cat#A12289; RRID:AB_2861647 HRP-conjugated Goat anti-Rabbit IgG ABclonal Cat#AS014; RRID:AB_2769854 Bacterial and virus strains Salmonella Typhimurium VNP20009 (VNP) ATCC Cat#202165 VNP-2 (VNP strains constitutively expresses RFP) This paper N/A DLV (VNP strains defective in lon gene) This paper N/A DLV-1(DLV strains constitutively expresses sfGFP) This paper N/A DLV-2 (DLV strains constitutively expresses RFP) This paper N/A DLV-3(DLV strains constitutively expresses luxCDABE) This paper N/A DLV-4(DLV strains constitutively expresses Nirfp) This paper N/A DLV-5(DLV strains expresses RFP with the aid of the sifB promoter) This paper N/A DLV-6(DLV strains expresses lysE with the aid of the sifB promoter) This paper N/A Chemicals, peptides, and recombinant proteins Mycoplasma removal reagent Yeasen Cat#40607ES03 transfection reagents Yeasen Cat#40802ES08 fixable viability dye BD Cat#564407 Hoechst dye KeyGEN BioTECH Cat#KGA1804-10 (Continued on next page) 18 Cell Reports 45, 116918, February 24, 2026 .. REAGENT or RESOURCE SOURCE IDENTIFIER Near-infrared lipophilic carbocyanine tracer DiR KeyGEN BioTECH Cat#KGE2603-5 Rhodamine MACKLIN Cat#R817228 ICG MACKLIN Cat#I953656 Luciferase substrate Vazyme Cat#DD1210 H2DCFDA MedChemExpress Cat#HY-D0940 Triton X-100 Sigma-Aldrich Cat#93443 M-CSF Yeasen Cat#91109ES10 GM-CSF Diane Biologics Cat#PDEM100050 IL4 protein Bioworld Cat#BK0246 DMF MedChemExpress Cat#HY-17363 DSF MedChemExpress Cat#HY-B0240 TAK-242 Selleck Cat#S7455 TH1020 MedChemExpress Cat#HY-116961 anti-PD1 antibody MedChemExpress Cat#A2122 Critical commercial assays Uniclone One-Step Seamless Cloning Kit Genesand Cat#SC612 T4 DNA ligase Sangon Biotech Cat#B600511-0001 FreeZol Reagent Vazyme Cat#R711-01 Reverse transcription kit Thermo Scientific Cat#M16315 AceQ qPCR SYBR Green Master Mix Vazyme Cat#Q221-01 permeabilization and fixation kit BD Biosciences Cat#555028 LDH Cytotoxicity Assay Kit Solarbio Cat#BC0680 ABplex Mouse Cytokine 15-Plex Assay Kit Abclonal Cat#RK05203 HMGB1 ELISA kit BYabscience Cat#BYHS504658 Deposited data Data files for RNA-sequencing This paper GEO: GSE291580 Experimental models: Cell lines 4T1 ATCC Cat#CRL-2539 H22 BLUEFBIO Cat#BFN608007276 A20 ATCC Cat#TIB-208 Panc02 Servicebio Cat RNA sequencing:Article Title: Controlled pyroptosis of engineered macrophages enables biphasic antitumor via the release of oncolytic bacteria and inflammatory signals. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies FITC-conjuagted-anti-MHCII BD Biosciences Cat#562009; RRID:AB_10893593 FITC-conjuagted-anti-CD3 BD Biosciences Cat#561801; RRID:AB_10895388 FITC-conjuagted-anti-CD11b BD Biosciences Cat#561684; RRID:AB_10893796 FITC-conjuagted-anti-CD44 BD Biosciences Cat#553133; RRID:AB_2076224 PE-Cy7-conjuagted-anti-CD11c BD Biosciences Cat#558079; RRID:AB_647251 PE-Cy7-conjuagted-anti-CD8 BD Biosciences Cat#552877; RRID:AB_394506 PE-conjuagted-anti-CD8 BD Biosciences Cat#554857; RRID:AB_395558 PE-conjuagted-anti-Foxp3 BD Biosciences Cat#563101; RRID:AB_2738006 PerCP-Cy5.5-conjuagted-anti-CD4 BD Biosciences Cat#550954; RRID:AB_393977 BV421-conjuagted-anti-F4/80 BD Biosciences Cat#565411; RRID:AB_2734779 BV510-conjuagted-anti-CD45 BD Biosciences Cat#740131; RRID:AB_2739888 APC-conjuagted-anti-CD62L BD Biosciences Cat#553152; RRID:AB_398533 APC-conjuagted-anti-F4/80 BD Biosciences Cat#566787; RRID:AB_2869866 APC-conjuagted-anti-CD11b BD Biosciences Cat#553312; RRID:AB_398535 APC-conjuagted-anti-CD206 Thermo Fisher Scientific Cat#17-2061-82; RRID:AB_2637420 APC-conjuagted-anti-GzmB Thermo Fisher Scientific Cat#17-8898-82; RRID:AB_2688068 PE-conjuagted-anti-CD86 Thermo Fisher Scientific Cat#12-0862-82; RRID:AB_465768 OVA257-264 (SIINFEKL) peptide bound to H-2Kb Monoclonal Antibody-APC Thermo Fisher Scientific Cat#17-5743-80; RRID:AB_1311288 FITC-conjuagted-anti-CD45.1 Biolegend Cat#110705; RRID:AB_313494 Anti-GSDMD rabbit mAb Abcam Cat#ab209845; RRID:AB_2783550 Anti-tubulin rabbit mAb ABclonal Cat#A12289; RRID:AB_2861647 HRP-conjugated Goat anti-Rabbit IgG ABclonal Cat#AS014; RRID:AB_2769854 Bacterial and virus strains Salmonella Typhimurium VNP20009 (VNP) ATCC Cat#202165 VNP-2 (VNP strains constitutively expresses RFP) This paper N/A DLV (VNP strains defective in lon gene) This paper N/A DLV-1(DLV strains constitutively expresses sfGFP) This paper N/A DLV-2 (DLV strains constitutively expresses RFP) This paper N/A DLV-3(DLV strains constitutively expresses luxCDABE) This paper N/A DLV-4(DLV strains constitutively expresses Nirfp) This paper N/A DLV-5(DLV strains expresses RFP with the aid of the sifB promoter) This paper N/A DLV-6(DLV strains expresses lysE with the aid of the sifB promoter) This paper N/A Chemicals, peptides, and recombinant proteins Mycoplasma removal reagent Yeasen Cat#40607ES03 transfection reagents Yeasen Cat#40802ES08 fixable viability dye BD Cat#564407 Hoechst dye KeyGEN BioTECH Cat#KGA1804-10 (Continued on next page) 18 Cell Reports 45, 116918, February 24, 2026 .. REAGENT or RESOURCE SOURCE IDENTIFIER Near-infrared lipophilic carbocyanine tracer DiR KeyGEN BioTECH Cat#KGE2603-5 Rhodamine MACKLIN Cat#R817228 ICG MACKLIN Cat#I953656 Luciferase substrate Vazyme Cat#DD1210 H2DCFDA MedChemExpress Cat#HY-D0940 Triton X-100 Sigma-Aldrich Cat#93443 M-CSF Yeasen Cat#91109ES10 GM-CSF Diane Biologics Cat#PDEM100050 IL4 protein Bioworld Cat#BK0246 DMF MedChemExpress Cat#HY-17363 DSF MedChemExpress Cat#HY-B0240 TAK-242 Selleck Cat#S7455 TH1020 MedChemExpress Cat#HY-116961 anti-PD1 antibody MedChemExpress Cat#A2122 Critical commercial assays Uniclone One-Step Seamless Cloning Kit Genesand Cat#SC612 T4 DNA ligase Sangon Biotech Cat#B600511-0001 FreeZol Reagent Vazyme Cat#R711-01 Reverse transcription kit Thermo Scientific Cat#M16315 AceQ qPCR SYBR Green Master Mix Vazyme Cat#Q221-01 permeabilization and fixation kit BD Biosciences Cat#555028 LDH Cytotoxicity Assay Kit Solarbio Cat#BC0680 ABplex Mouse Cytokine 15-Plex Assay Kit Abclonal Cat#RK05203 HMGB1 ELISA kit BYabscience Cat#BYHS504658 Deposited data Data files for RNA-sequencing This paper GEO: GSE291580 Experimental models: Cell lines 4T1 ATCC Cat#CRL-2539 H22 BLUEFBIO Cat#BFN608007276 A20 ATCC Cat#TIB-208 Panc02 Servicebio Cat |